Journal: bioRxiv

Article Title: Hypercholesterolemia aggravates in-stent restenosis in rabbits: a mitigating effect of stent surface modification with CD47-derived peptide

doi: 10.1101/2023.02.27.530304

Figure Lengend Snippet: (A-P) Representative microscopic images (100x magnification) of iliac arteries stented with BMS (A, B, E, F, I, J, M, N) and pepCD47-functionalized stents (C, G, K, O) and immunostained for TNFα (A-C), IL1β (E-G), IL6 (I-K) and CD68-positive macrophages (M-O). Quantification of TNFα (D), IL1β (H), and IL6 (L) expression was based on a semi-quantitative (0-4) scale. Macrophage infiltration around the struts (P) was calculated as a percentage of a strut circumference occupied with the CD68-positive cells (n=4 for each marker/treatment group combination).

Article Snippet: Primary antibodies to rabbit CD68, TNFα, IL1β, and IL6 were from ProSci (Poway, CA, USA), MyBioSource (San Diego, CAS, USA), Bioss (Woburn, MA, USA), and Abbexa (Cambridge, UK), respectively.

Techniques: Expressing, Marker

Journal: Scientific Reports

Article Title: Quercetin can reduce viral RNA level of O’nyong-nyong virus and resulting innate immune cytokine responses in cultured human synovial fibroblasts

doi: 10.1038/s41598-021-85840-z

Figure Lengend Snippet: Concentration-dependent cytotoxic effects of phenolics or ONNV on cultured HSF cells. Cells were infected by ONNV (MOI 10 –3 to 1) or phenolics (1 to 100 µM) for 24 h. Released LDH in culture medium was measured using a colorimetric-based method (CytoTox 96 Non-Radioactive Cytotoxicity Assay) and expressed relative to the maximum release by application of a lysis buffer (Triton 1%). Reported values are means ± SEM of three independent experiments and p value was calculated using the Bonferroni multiple comparison test (** p < 0.01; *** p < 0.001) with untreated cells (CTRL).

Article Snippet: HSF cells were obtained from ScienCell research laboratory (ScienCell, 4700 Clinisciences) and were grown in Modified Eagle's Medium (MEM, PAN Biotech P0408500) supplemented with 10% heat-inactivated fetal bovine serum (FBS; PAN Biotech, 3302 P290907), 2 mM of L-glutamine (Biochrom AG, K0282), 0.1 mg/mL penicillin–streptomycin (PAN Biotech, P0607100), 1 mM of sodium pyruvate (PAN Biotech, P0443100) and 0,5 μg/mL of amphotericin B (PAN Biotech, P0601001).

Techniques: Concentration Assay, Cell Culture, Infection, Cytotoxicity Assay, Lysis, Comparison

Journal: Scientific Reports

Article Title: Quercetin can reduce viral RNA level of O’nyong-nyong virus and resulting innate immune cytokine responses in cultured human synovial fibroblasts

doi: 10.1038/s41598-021-85840-z

Figure Lengend Snippet: Quercetin impairs HSF cells infection by ONNV. HSF cells were exposed to ONNV (MOI 1) co-treated or not with phenolics (10 µM) for 24 h. Cells were probed with primary antibody Alpha SC 58,088 anti-alphavirus before nuclei staining by DAPI (blue) and antibody binding by Alexa Fluor 488-conjugated donkey anti-mouse IgG antibody (green) ( A ). Untreated cells (CTRL) and infected cells (+ ONNV) ( B ) Alexa Fluor 488/DAPI overlay upon phenolic treatment. ( C ) Ratio Alexa Fluor 488 / DAPI fluorescence intensity with at least 10,000 DAPI stained cells. Reported values are means ± SEM of three independent experiments. p value was calculated using the Bonferroni multiple comparison test (*** p < 0.001) between infected cells (+ ONNV) and untreated cells (CTRL) or ( ## p < 0.01, ### p < 0.001) between infected cells (+ ONNV) and infected cells upon treatment with phenolics.

Article Snippet: HSF cells were obtained from ScienCell research laboratory (ScienCell, 4700 Clinisciences) and were grown in Modified Eagle's Medium (MEM, PAN Biotech P0408500) supplemented with 10% heat-inactivated fetal bovine serum (FBS; PAN Biotech, 3302 P290907), 2 mM of L-glutamine (Biochrom AG, K0282), 0.1 mg/mL penicillin–streptomycin (PAN Biotech, P0607100), 1 mM of sodium pyruvate (PAN Biotech, P0443100) and 0,5 μg/mL of amphotericin B (PAN Biotech, P0601001).

Techniques: Infection, Staining, Binding Assay, Fluorescence, Comparison

Journal: Scientific Reports

Article Title: Quercetin can reduce viral RNA level of O’nyong-nyong virus and resulting innate immune cytokine responses in cultured human synovial fibroblasts

doi: 10.1038/s41598-021-85840-z

Figure Lengend Snippet: Quercetin impairs ONNV replication in HSF cells. HSF cells were infected by ONNV (MOI 1) co-treated or not with phenolic compounds (10 µM) for 24 h. Then, RNA was collected and E1, E2, nsP1 and nsP2 gene expression levels were determined by qRT-PCR. Reported values are means ± SEM of three independent experiments. p value was calculated using the Bonferroni multiple comparison test: *p < 0.05, **p < 0.01, ***p < 0.001 when compared to non-infected and non-treated cells (CTRL) and # p < 0.05, ## p < 0.01, ### p < 0.001 when compared to infected cells (+ ONNV).

Article Snippet: HSF cells were obtained from ScienCell research laboratory (ScienCell, 4700 Clinisciences) and were grown in Modified Eagle's Medium (MEM, PAN Biotech P0408500) supplemented with 10% heat-inactivated fetal bovine serum (FBS; PAN Biotech, 3302 P290907), 2 mM of L-glutamine (Biochrom AG, K0282), 0.1 mg/mL penicillin–streptomycin (PAN Biotech, P0607100), 1 mM of sodium pyruvate (PAN Biotech, P0443100) and 0,5 μg/mL of amphotericin B (PAN Biotech, P0601001).

Techniques: Infection, Gene Expression, Quantitative RT-PCR, Comparison

Journal: Scientific Reports

Article Title: Quercetin can reduce viral RNA level of O’nyong-nyong virus and resulting innate immune cytokine responses in cultured human synovial fibroblasts

doi: 10.1038/s41598-021-85840-z

Figure Lengend Snippet: Quercetin impairs ONNV replication in supernatants in HSF cells. HSF cells were infected by ONNV (MOI 1) co-treated or not with phenolic compounds (10 µM) for 24 h. Then, RNA was collected in the supernatants and E1, E2, nsP1 and nsP2 gene expression levels were determined by qRT-PCR. Reported values are means ± SEM of three independent experiments. p value was calculated using the Bonferroni multiple comparison test: *p < 0.05, **p < 0.01, ***p < 0.001 when compared to non-infected and non-treated cells (CTRL) and # p < 0.05, ## p < 0.01 when compared to infected cells (+ ONNV).

Article Snippet: HSF cells were obtained from ScienCell research laboratory (ScienCell, 4700 Clinisciences) and were grown in Modified Eagle's Medium (MEM, PAN Biotech P0408500) supplemented with 10% heat-inactivated fetal bovine serum (FBS; PAN Biotech, 3302 P290907), 2 mM of L-glutamine (Biochrom AG, K0282), 0.1 mg/mL penicillin–streptomycin (PAN Biotech, P0607100), 1 mM of sodium pyruvate (PAN Biotech, P0443100) and 0,5 μg/mL of amphotericin B (PAN Biotech, P0601001).

Techniques: Infection, Gene Expression, Quantitative RT-PCR, Comparison

Journal: Scientific Reports

Article Title: Quercetin can reduce viral RNA level of O’nyong-nyong virus and resulting innate immune cytokine responses in cultured human synovial fibroblasts

doi: 10.1038/s41598-021-85840-z

Figure Lengend Snippet: Quercetin reduces CCL-2 and IL-1β pro-inflammatory mediators production in ONNV infected HSF cells. HSF cells were infected by ONNV (MOI 1) and/or treated with phenolics (10 µM) for 24 h. Then, RNA was collected and CCL-2 and IL-1β gene expression levels were determined by qRT-PCR, ( A ) Pro-inflammatory mediators RNA levels. Then, culture media were collected CCL-2 and IL-1β levels evaluated by ELISA, ( B ) Pro-inflammatory mediators levels. Reported values are means ± SEM of three independent experiments. p value was calculated using the Bonferroni multiple comparison test: *p < 0.05, **p < 0.01, ***p < 0.001 when compared to non-infected and non-treated cells (CTRL) and # p < 0.05, ## p < 0.01, ### p < 0.001 when compared to infected cells (+ ONNV).

Article Snippet: HSF cells were obtained from ScienCell research laboratory (ScienCell, 4700 Clinisciences) and were grown in Modified Eagle's Medium (MEM, PAN Biotech P0408500) supplemented with 10% heat-inactivated fetal bovine serum (FBS; PAN Biotech, 3302 P290907), 2 mM of L-glutamine (Biochrom AG, K0282), 0.1 mg/mL penicillin–streptomycin (PAN Biotech, P0607100), 1 mM of sodium pyruvate (PAN Biotech, P0443100) and 0,5 μg/mL of amphotericin B (PAN Biotech, P0601001).

Techniques: Infection, Gene Expression, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Comparison